This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.
Highlights:
*Shows reactivity against VSV-G pseudotyped lentiviruses (see references below)
*Suitable for Western Blot, Flow Cytometry and Immunofluorescence applications
*Fromthe hybridoma 8G5F11
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.
| Product Type: | Antibody |
| Name: | Anti-VSV-G [8G5F11] |
| Antigen: | VSV-G |
| Accession ID: | P03522 |
| Host: | Mouse |
| Isotype: | EB0010: IgG2a kappa Recombinant versions: see product name |
| Clonality: | Monoclonal |
| Clone Name: | 8G5FL11 (I1) |
| Specificity: | VSV-Ind glycoprotein (G) protein |
| Reactivity: | Human |
| Immunogen: | VSV infection |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Buffer: | EB0010: PBS, 0.05% (w/v) Sodium Azide Recombinant versions: PBS with 0.02% Proclin 301 |
| Tested Applications: | Western blot (1:1000), flow cytometry, immunofluorescence |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | -20C (avoid repeated freeze / thaw cycles) |
| Shipped: | Cold packs |
![Kerafast代理EB0010現(xiàn)貨Anti-VSV-G [8G5F11] Antibody操作使用](https://img69.chem17.com/2/20200506/637244041000013126875.jpg)
參考文獻：
1. Gregory DA, Olinger GY, Lucas TM, Johnson MC. Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles. Retrovirology. 2014 Apr 3;11(1):28.
2. Kubo S, Mitani K. A New Hybrid System Capable of Efficient Lentiviral Vector Production and Stable Gene Transfer Mediated by a Single Helper-Dependent Adenoviral Vector. JVirol 77: 2964-2971, 2003.
3. Kobayashi M, Iida A, Ueda Y, and Hasegawa M. Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus. JVirol 77: 2607-2614, 2003.
4. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. PNAS 90: 8033-8037, 1993.
5. Vandepol SB, Lefrancois L, Holland JJ. Sequences of the Major Anitbody Binding Epitopes of the Indiana Serotype of Vesicular Stomatitis Virus. Virology 148: 312-325 (1986).
6. Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982.
